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SPECIES: mouse, C3H
ECACC catalog code: 91031101
Description: Mouse C3H muscle myoblast
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Differentiation studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 10-15% Fetal Bovine Serum (FBS). Split semi-confluent cultures 1:3 to 1:6 i.e. seeding at 1-2x1,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C .Do not allow cells to reach confluence.
Subclone from myoblast line established from normal adult C3H mouse leg muscle. Differentiates rapidly; produces extensive contracting myotubes expressing characteristic muscle proteins. Provides model to study in vitro myogenesis and cell differentiation.
Research council deposit: No;
Price Code: A;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CRL 1772, IZSBS: BS CL 148
- Nature 1977;270:725-727 - PMID: 563524
- Science 1985;230:758-766 - PMID: 2414846
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.