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ECACC catalog code: 94101907
Description: Rat hepatoma [HGPRT-]
Viruses: See Properties
Depositor: Dr M Weiss, Dept. Biologie Moleculaire, Institute Pasteur, Paris, FRANCE (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Products: Albumin and hepatocyte markers Applications: Gene expression studies.
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
Coon's modified Ham's F12 + 2mM Glutamine + 5% Fetal Bovine Serum (FBS). Split semi-confluent cultures (70%) 1:10 i.e. seeding at 9x1000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C. Saturation density is reached at 15x1,000,000 cells/10cm² dish.
Karyotype: Hyperdiploid, 51 chromosomes
C2-Rev 7 is a revertant of the C2 variants, selected by virtue of its capacity to grow in glucose free medium (containing dialysed serum) where survival requires activity of the liver-specific gluconeogenic pathway. Expression of the entire set of liver specific functions is restored. Albumin and hepatocyte markers present.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species ocnfirmation)
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.