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TISSUE/ORGAN: peripheral blood
TUMOR: leukemia, chronic myeloid
DSMZ catalog code: ACC 270
Morphology: round cells growing singly or in small clusters in suspension
Description: human chronic myeloid leukemia in blast crisis (derivative of LAMA-84) the parental cell line LAMA-84 (DSM ACC 168) has been established from the peripheral blood of a 29-year-old woman with chronic myeloid leukemia (CML) in blast crisis; subclone LAMA-87 was obtained after subcutaneous transplantation of LAMA-84 cells into estrone-treated nude mice confirmed as human with IEF of LDH, NP
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HIV-, HTLV-I/II-
Depositor: Dr. P. Champelovier, Centre Hospitalier Universitaire, Grenoble, France
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
80% RPMI 1640 + 20% FBS split saturated culture 1:2 every 2-3 days; spent medium should be exchanged every 24-48 hours even without splitting of the cells; seed out at ca. 0.5 x 106 cells/ml at 37C with 5% CO2 maximal density at ca. 1 x 10 6 cells/ml;
doubling time of ca. 50-60 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 ("human near-triploid karyotype; 68<3n>XX-Y, -8, -9, +11, -13, -13, +15, -20, -21, -22, +4mar; del(1)(p35), del(3)(p25), del(4)(q27q31), del(5)(q32), add(6)(q24), del(7)(p15), i(13q), add(14)(p11), del(17)(p13), add(19)(p13); 19p13) * cytogenetics 2 (recurrent breakpoint in gastric carcinoma; similar to published karyotype) * DNA fingerprinting (unique DNA profile with (gtg)5 multilocus probe) * immunological analysis (cytokeratin+ desmin- endothel- GFAP- neurofilament- vimentin-) * isoenzymes (confirmed as human with IEF of MDH, NP) * reverse transcriptase (reverse transcriptase not detected)
- Virchows Arch B Cell Pathol 1993;63:335-343
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.