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IZSBS catalog code: BS TCL 6
Continuous culture, grown as monolayer
SPECIES: mouse, RIII Karyology: unknown
TISSUE/ORGAN: mammary gland
TUMOR: mammary tumor
Susceptibility to viruses: bovine papilloma * sarcoma virus
DEPOSITOR: obtained from ATCC
PROPERTIES: virology; transformation
Available in the following LABORATORY:
- Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna (IZSBS, Brescia)
DMEM + 10% FBS; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2 Passage: 5, mycoplasma negative, culture
Cell line has been employed as a suitable host for transformation with bovine papilloma virus DNA plasmids. The cells have been used for visualization of sarcoma virus and for in vi quantitative assay for bovine papilloma virus.
Validated by: isoenzymes (confirmed as mouse with NP, G6PD, MD, PEPB, LD)
Availability in cell line catalogues: ATCC: CRL 1616
- J Virol 1978;26:291-298 - PMID: 26810
- Mol Cell Biol 1981;1:486-496 - PMID: 6100967
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.