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ICLC catalog code: HTL01004
Continuous culture, grown as suspension
lymphoblastic, cells growing single and in clusters morphology
SPECIES: human, Caucasian (female, 4 years old) Karyology: approx. tetraploid
TUMOR: leukemia, acute lymphoblastic T cell
DEPOSITOR: DAKO S.p.A., Milan, Italy; DAKO A/S, Glostrup, Denmark
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: The cell line has very long telomeres
Available in the following LABORATORY:
- IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
RPMI 1640 + 10% FBS + 2mM L-Glutamine; Freezing medium: 90% FBS + 10% DMSO; maintain cultures between 2 and 9x10^5 cells/ml; split 1:5-1:10 every 2 to 3 days; 37C, 5% CO2, mycoplasma negative, HOECHST and PCR
Validated by: HLA-DP beta (confirmed as human by PCR)
- Dev Biol Stand 1979;42:193-197 - PMID: 223921
- Nucleic Acids Res. 1998;26(16):3651-6 - PMID: 9685479
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.