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TISSUE/ORGAN: bone marrow
TUMOR: leukemia, acute myeloid
DSMZ catalog code: ACC 137
Morphology: round (relatively large) cells growing singly in suspension (ca. 1-2% cells are slightly adherent)
Description: human acute myeloid leukemia established from the bone marrow of a 64-year-old man with acute myeloid leukemia (AML M7) at diagnosis in 1988; cells are constitutively cytokine-dependent and responsive to various cytokines confirmed as human with IEF of LDH, NP
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HIV-, HTLV-I/II-
Depositor: Dr. H. G. Drexler, DSMZ, Braunschweig, Germany
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
80% alpha-MEM + 20% FBS + 5 ng/ml GM-CSF maintain at 0.5-1.0 x 106 cells/ml; cultivate in 12- or 24-well-plate for optimal growth; split saturated culture 1:2 every 2-3 days; seed out at ca. 0.5 x 106 cells/ml at 37C with 5% CO2 saturation density at ca. 1.0-1.5 x 106 cells/ml;
doubling time of ca. 60-70 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 4-5 x10 6 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.