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SPECIES: human, Caucasian
TUMOR: carcinoma, cervical
ECACC catalog code: 89051702
Description: Human Caucasian Cervical carcinoma
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Differentiation studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
Waymouth's MB 752/1 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:4 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Karyotype: 2n = 46, hypodiploid
Established from cervical carcinoma of 41 year old Caucasian female. Cells form stratified, epithelial colonies and retain properties of squamous cell differentiation in long term culture. They are morphologically and cytogenetically distinct from C-4 II (ECACC No. 88102701). Reported to contain human papilloma virus (Am. J. Pathol. 119:361-366). Handle as potentially Biohazardous Material.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CRL 1594
- J Natl Cancer Inst 1962;28:605-627
- J Natl Cancer Inst 1969;43:151-173 - PMID: 5796380
- J Natl Cancer Inst 1969;43:175-190 - PMID: 5796382
- J Natl Cancer Inst 1972;48:1589 - PMID: 5056250
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.