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IZSBS catalog code: BS TCL 125
Continuous culture, grown as monolayer
SPECIES: human, Caucasian (female, 68 years old) Karyology: 2n = 46 in 32 cells
TUMOR: astrocytoma, anaplastic, grade IV
DEPOSITOR: obtained from ATCC
PROPERTIES: Model for multiple sclerosis
Available in the following LABORATORY:
- Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna (IZSBS, Brescia)
RPMI 1640 + 10% FBS; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2 Passage: 15, mycoplasma negative, culture
Presenting acid phosphatase activity and glial fibrillary acid protein in 70-80% of the cells. HLA-DR is detected in 20-30% of the cells.
Validated by: isoenzymes (confirmed as human with NP, G6PD, AST, LD)
Availability in cell line catalogues: ATCC: CRL-1718, ECACC: 90021502
- Experimental Allergic Encephalomyelitis, A R Liss Inc 1984;153
- Fed Proc 1984;43:781
- In Vitro 1983;19:275
- J Neuroimmunol 1985;10:151 - PMID: 3877740
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.