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IZSBS catalog code: BS CL 148
Continuous culture, grown as monolayer
DEPOSITOR: obtained from ATCC
PROPERTIES: used in studies of myogenesis in vitro and cell differentiation
Available in the following LABORATORY:
- Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna (IZSBS, Brescia)
DMEM + 10% FBS; Freezing medium: Culture medium + 5% DMSO; 37C, 5% CO2, mycoplasma negative, culture
Do not allow the culture to reach confluence because differentiates rapidly and produces extensive contracting myotubes expressing characteristic muscle proteins.
Validated by: isoenzymes (confirmed as mouse with NP, G6PD, AST, LD)
Availability in cell line catalogues: ATCC: CRL-1772, ECACC: 91031101
- Nature 1977;270:725-727 - PMID: 563524
- Science 1985;230:758-766 - PMID: 2414846
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.