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TISSUE/ORGAN: lung, fetal
ECACC catalog code: 97112601
Description: Human fetal lung
Viruses: See Properties
Depositor: Dr P Jacobs, NIBSC, London, UK
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Virus studies.
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10mM Hepes + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; Hepes; 37C.
Karyotype: 2n = 46, diploid
Established from normal lung tissue of a 14 week old male foetus. The cells undergo between 60-70 population doublings before senescence. The virus susceptibility of this line is similar to WI-38. This cell line is supplied on a standing order basis.
Research council deposit: No;
Price Code: G;
Availability in cell line catalogues: ICLC: HL95001
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.