This is the complete description of the cell line that you selected.
By clicking on a generic term (e.g., SPECIES) you will go to the corresponding index (e.g., index of species), while by clicking on a specific term (e.g., retinoblastoma) you will go to the list of corresponding cell lines (e.g., retionoblastoma cell lines).
IZSBS catalog code: CL 142
Continuous culture, grown as monolayer
DEPOSITOR: obtained from ATCC
PROPERTIES: producing an extracellular matrix that improves the growth of neuronal cells and astrocytes
Available in the following LABORATORY:
- Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna (IZSBS, Brescia)
DMEM + 15% FBS; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2 Passage: 75, mycoplasma negative, culture
The cell doubling time decreases with passages and can be increased by reducing FBS (2.5%) and adding calf serum (5%)
Validated by: isoenzymes (confirmed as bovine with NP, G6PD, AST)
Availability in cell line catalogues: ATCC: CRL-2048
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.