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SPECIES: mouse, AKR/J
ECACC catalog code: 85082302
Description: Mouse AKR/J T cell lymphoma
Viruses: See Properties
Depositor: Dr P Marrack/Dr J Kappler, National Jewish Center, Denver, USA (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Fusion partner
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
SMEM + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 2mM Sodium Pyruvate (NaP) + 0.05mM 2-Mercaptoethanol (2ME) + 10% Fetal Bovine Serum (FBS). Maintain cultures between 1-6x100,000 cells/ml; 5% CO2; 37C.
This line is a mutant sub-line of BW5147 which was derived from an AKR/J mouse lymphoma. The cells are resistant to 100ÁM thioguanine and 1mM ouabain. The cells can be used to produce T-cell hybrids.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
- Cell 1978;14:21 - PMID: 667934
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.