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SPECIES: mouse / mouse
DSMZ catalog code: ACC 183
Morphology: single cells growing in suspension
Description: mouse hybridoma (anti-adenovirus 2) established by fusion of SP2/0-AG14 myeloma cells with spleen cells from BALB/c mice immunized with HELA cells that were infected with adenovirus 2; cells secrete an IgG monoclonal antibody reacting with an adenovirus group specific antigen; cell line is patented confirmed as mouse with IEF of AST, NP, PEP B
Viruses: ELISA: reverse transcriptase negative
Depositor: Dr. H. G. Drexler, DSMZ, Braunschweig, Germany
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% Dulbecco's MEM (4.5 g/L glucose) + 10% FBS optimal split ratio of 1:2 every 2-3 days at 37C with 5% CO2 cell harvest of about 1-2 x 106 cells/ml;
frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (murine karyotype with centric fusion and 16% polyploidy: 66(58-74)<3n>) * isoenzymes (confirmed as mouse with IEF of AST, NP, PEP B) * reverse transcriptase (reverse transcriptase negative)
Availability in cell line catalogues: ATCC: HB 8117
- Virology 1981;110:385-401 - PMID: 7222481
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.