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SPECIES: human, Caucasian
TISSUE/ORGAN: lung, metastasis
TUMOR: carcinoma, embryonal pluripotent
ECACC catalog code: 94081613
Description: Human Caucasian pluripotent embryonal carcinoma
Viruses: See Properties
Depositor: Professor P W Andrews, Department Biomedical Science, University of Sheffield, UK (Originator)
Restricted. Patent: None Specified By Depositor
Properties: Applications: Regulation of human embryonic differentiation, neuronal studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM containing 4.5 g/L glucose + 2mM glutamine + 10% FBS Split confluent cultures 1:3 to 1:4 i.e. seeding 3-5 x 10,000 cells / cm2; harvest cells by scraping and maintain continuously at high density
The pluripotent human embryonal carcinoma cell line NTERA-2 clone D1, also known as NT2/D1, is a subclone derived from the parent line NTERA-2. NTERA-2 cells were established from a nude mouse xenograft tumour of TERA-2 cells, which were originally isolated from a lung metastasis from a 22 year old patient with primary embryonal carcinoma of the testis. The subline NTERA-2 clone D1 differentiates into neuronal and other cell types in response to retinoic acid (RA) or hexamethylene bisacetamide (HMBA). Differentiated cells become permissive for human cytomegalovirus (HCMV) or human immunodeficiency virus (HIV). Differentiation with RA as inducer results in glycolipid changes, appearance of neurons and induction of Homeobox (HOX) gene clusters.
Research council deposit: No;
Price Code: C;
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.