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SPECIES: human, Caucasian
TUMOR: adenocarcinoma, endometrial
ECACC catalog code: 98050131
Description: Human caucasian endometrial adenocarcinoma
Viruses: See Properties
Depositor: Dr R Hackenberg, Universitšt-Frauenklinik, Marburg, Germany, GERMANY (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Products: Placental protein 14 Applications: Tumour biology studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
MEM + 2mM glutamine + 10% FBS Split confluent cultures 1:3 i.e. seeding 2-4 x 10,000 cells / cm2 using 0.25% trypsin or trypsin / EDTA; 5% CO2; 37C
Karyotype: Near diploid
MFE-280 was derived from a recurrent, poorly differentiated endometrial carcinoma from a 78 year old patient. Initially, cells were grown as aggregates in suspension for one year, after which they were transferred to monolayer culture. The karyotype analysis revealed near-diploidy with a complex heterogeneous aberration pattern. MFE-280 were found to be positive for cytokines 7, 8, 18 and 19 as well as for vimentin. In addition, the cells express the placental protein 14, PP14. They have been shown to be tumorigenic in nude mice. This cell line has only been subject to preliminary quality control procedures. Authentication tests such as DNA fingerprinting will be performed only when this cell line is requested.
Research council deposit: No;
Price Code: C;
Availability in cell line catalogues: DSMZ: ACC 410
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.