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SPECIES: human, Caucasian
TUMOR: leukemia, promyelocytic
ECACC catalog code: 96100920
Description: Human Caucasian promyelocytic leukaemia
Viruses: See Properties
Depositor: Dr D W Golde, Memorial Sloan-Kettering Cancer Centre, New York, USA (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Study of production and function of human eosinophils, analysis of differentiation.
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
IDMEM + 2mM Glutamine + 0.5mM Butyric acid + 10% Fetal Bovine Serum (FBS); pH7.6; add Butyric acid to the medium just before use. Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37C.
Eos-HL-60 is a variant of the promyelocytic cell line HL60 (ECACC No. 85011431) established by sub-cloning in methylcellulose. Although the cells are capable of reverting to the parental phenotype, most passages maintain a high degree of eosinophil differentiation.
Research council deposit: No;
Price Code: C;
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.