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TUMOR: hybridoma, B cell
ECACC catalog code: 96080128
Description: Mouse B cell hybridoma
Viruses: See Properties
Depositor: Professor L A Aarden, Department Autoimmune Diseases, Central Laboratory of the Netherlands Red Cross Blood Transfusion Service, Amsterdam, The Netherlands, NETHERLANDS (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Bioassay for IL-6
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
IDMEM or RPMI 1640 + 2mM glutamine + 50ÁM 2ME + 50pg/ml rhu IL-6 + 5% FBS Maintain cultures between 3-9x100,000 cells/ml;5% CO2;37C
The B cell hybridoma B9 has been cloned from the parent line B13.29 and is highly dependent on IL-6 for growth. The cells have been applied in bioassays for IL-6 of human, bovine, horse, pig, dog, rat, mouse and seal origin. For routine culture, cells are grown in 10-100 units / ml IL-6. This cell line has only been subject to preliminary quality control procedures. Authentication tests such as DNA fingerprinting will be performed only when this cell line is requested.
Research council deposit: No;
Price Code: C;
Availability in cell line catalogues: DSMZ: ACC 211, ICLC: HYL98004
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.