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SPECIES: insect - Estigmene agrea
DSMZ catalog code: ACC 176
Morphology: very large cells growing in suspension; most cells are round to oval, 10% have long processes and are spindle shaped
Description: insect - Estigmene agrea established from the larvae of Estigmene agrea (family Arctiidae, order Lepidoptera) (Baerenspinner) confirmed as not murine and not primate with IEF of AST, PEP B
Depositor: Dr. H. S. Conradt, GBF, Braunschweig, Germany
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
80% TC 100 medium + 20% FBS cells are difficult to culture and grow slowly! split saturated culture 1:4 to 1:5 every 4-5 days no CO2 (keep lid of flask closed) at room temperature (20-25C) cell harvest of about 0.5 x 106 cells/ml;
frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: isoenzymes (confirmed as not murine and not primate with IEF of AST, PEP B)
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.