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SPECIES: human, Caucasian
DSMZ catalog code: ACC 227
Morphology: epitheloid cells growing adherently in monolayers
Description: human cervix carcinoma (derivative of HELA) these cells were thought to be 'stablished from the malignant urinary bladder carcinoma of a 66-year-old Caucasian man (stage pTa G3) in 1989'; same donor as for cell line BT-A; however, DNA fingerprinting and cytogenetic analysis at the DSMZ established unequivocally that this cell line must be considered de facto a subclone of HELA confirmed as human with IEF of AST, NP, PEP B
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HIV-, HTLV-I/II-
Depositor: Prof. A. J. Ulmer, Forschungsinstitut Borstel, Borstel, Germany
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS split confluent culture 1:5 to 1:10 every 3-4 days using trypsin/EDTA at 37C with 5% CO2 cell harvest of about 0.2-0.3 x 106 cells/cm 2;
doubling time of about 43 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 106 cells/ampoule;
contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (human hypertriploid karyotype with 13% polyploidy; 72(68- 74)<3n>XX, -Y, +1, -4, +5, +5, +5, -11, +12, +12, -19, -21, -22, +3 mar, der(1)t(1;3)(p11;q11), der(1)t(1;5)(p21;q21), der(2)t(2;5)(p11;q11), del(3)(q13), add(4)(q35), del 5)(p12),) * cytogenetics 2 (i(5p)x3, add(6)(q25/27), i(7q), del(8)(q23), del(10)(p11), inv(12)(q21q24)/der(12)t(12;?)(q21;?)x2, add(19)(p13); mar1 resembles i(Xp)) * DNA fingerprinting (unique, homozygous profile with pYNH24 probe) * immunological analysis (cytokeratin+ (70%), desmin-, endothel-, GFAP-, neurofilament+ (30-50%), vimentin+ (100%)) * isoenzymes (confirmed as human with IEF of AST, NP, PEP B) * reverse transcriptase (reverse transcriptase not detected)
- Exp Cell Res 1990;187:185-192 - PMID: 2105047
- Immunol Lett 1991;27:191 - PMID: 2060970
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.