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SPECIES: mouse / mouse
DSMZ catalog code: ACC 178
Morphology: single cells in suspension
Description: mouse hybridoma (anti-canine adenovirus type 1) established by fusion of SP2/0-AG14 myeloma cells with BALB/c mouse spleen cells immunized with purified canine adenovirus type 1 (CAV-1); the secreted antibody binds to and neutralizes CAV-1 and is type-specific confirmed as mouse with IEF of AST, NP
Viruses: ELISA: reverse transcriptase negative
Depositor: Dr. H. G. Drexler, DSMZ, Braunschweig, Germany
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
92.5% Dulbecco's MEM (4.5 g/L glucose) + 5% horse serum + 2.5% FBS maintain at 0.5-1.0 x 106 cells/ml; optimal split ratio of 1:2 every 2-3 days at 37C with 5% CO2 maximum density at about 1-2 x 106 cells/ml;
frozen with 70% medium, 10% horse serum, 5% FBS, 10% DMSO at about 5 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (92(88-101)<4n>; murine hypertetraploid karyotype with centric fusion and 1.7% polyploidy) * isoenzymes (confirmed as mouse with IEF of AST, NP) * reverse transcriptase (reverse transcriptase negative)
Availability in cell line catalogues: ATCC: CRL 1812
- Intervirology 1985;23:116-120 - PMID: 3884549
- Vet Microbiol 1988;16:1-8 - PMID: 3281371
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.