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ICLC catalog code: HTL00006
Continuous culture, grown as suspension
round and oval cells morphology
SPECIES: human (female, 6 years old)
TISSUE/ORGAN: pleural effusion
TUMOR: leukemia, T cell
DEPOSITOR: Dr L. Pegoraro, Cytogenetics and Molecular Biology Dept, University of Turin, Italy
Cell line available for distribution. For non-commercial investigative use only
Available in the following LABORATORY:
- IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
RPMI 1640 + 10-20% FBS + 2mM L-Glutamine; Freezing medium: 90% FBS + 10% DMSO; maintain cultures between 0.5-2 x 10^6 cells/ml; split 1:2-1:5; 37C, 5% CO2, mycoplasma negative, HOECHST and PCR
The cells should be frozen at not less than 5 x 10^6 cells/ampoule. The viability may be low after thawing; thaw with medium with 20% FBS
Validated by: HLA-DP beta (confirmed as human by PCR)
Availability in cell line catalogues: DSMZ: ACC 38
- J Natl Cancer Inst 1985;75:285-290 - PMID: 2410652
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.