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ICLC catalog code: HTL99029
Continuous culture, grown as suspension
multicellular clusters morphology
SPECIES: human, Caucasian (female, 2 years old)
DEPOSITOR: Dr F. Tosetti, Molecular Biology Dept, IST at ABC, Genoa, Italy
Cell line available for distribution. For non-commercial investigative use only
Available in the following LABORATORY:
- IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
RPMI 1640 + 2mM L-Glutamine + 15% FBS; Freezing medium: 90% FBS + 10% DMSO; maintain cultures between 5-9 x 10^5 cells/ml; split 1:2-1:4 (not disaggregate the clusters); 37C, 5% CO2. After thawing the viability of the cells may be low (thaw with medium supplemented with 20% FBS)., mycoplasma negative, HOECHST and PCR
In order to obtain a cell monolayer the surface of the flask must be coated with poly-D-lysine 2.5 ug/cm2
Validated by: HLA-DP beta (confirmed as human by PCR)
Availability in cell line catalogues: ECACC: 86093003
- J Natl Cancer Inst 1974;53:347-360 - PMID: 4135597
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.