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ICLC catalog code: ATL99013
Continuous culture, grown as monolayer
SPECIES: mouse, C3H/He
DEPOSITOR: Dr F. Tosetti, Molecular Biology Dept, IST at ABC, Genoa, Italy
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: P19 cells can be induced by retinoic acid (RA) to differentiate into neuronal and glial cells or contractile cardiac muscle cells, depending upon RA concentration and cell density. They can also spontaneously differentiate when plated at too low or too high density and when grown in suspension.
Available in the following LABORATORY:
- IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
DMEM + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; split confluent cultures 1:10-1:20 using trypsin/EDTA; seed at 2-4 x 10^4 cells/cm2; 37C, 5% CO2, mycoplasma contamination eliminated with MRA; negative, HOECHST and PCR
Validated by: isoenzymes (confirmed as mouse with NP, PepB)
Availability in cell line catalogues: ECACC: 95102107
- Dev Biol 1982;89:503-508 - PMID: 82117859
- J Cell Biol 1982;94:253-262 - PMID: 82265903
- Mol Cell Biol 1983;3:2271-2279 - PMID: 6656766
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.