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ICLC catalog code: HTL99020
Continuous culture, grown as monolayer
SPECIES: human, Caucasian (female, 82 years old)
TUMOR: adenocarcinoma, grade IV
tumorigenic in nude mice
DEPOSITOR: Dr L. Varesco, Exp. Oncology Dept, IST, Genoa, Italy
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: HLA: A23, A33; B27, B35
Available in the following LABORATORY:
- IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
L-15 + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; split confluent culture 1:3-1:4 using trypsin/EDTA; seed at 2-4 x 10^4 cells/cm2; incubate at 37°C without CO2 (close the flask caps), mycoplasma negative, HOECHST and PCR
the cells are difficult to detach: at least 15 min. in trypsin or scraper are required
Validated by: HLA-DP beta (confirmed as human by PCR)
- Cancer Res 1976;36:4562-4569 - PMID: 1000501
- J Natl Cancer Inst 1977;59:221-226 - PMID: 327080
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.