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ICLC catalog code: HTL99018
Continuous culture, grown as suspension
small round cells morphology
SPECIES: human (male, 45 years old)
TISSUE/ORGAN: peripheral blood
TUMOR: leukemia, pre-B cell
Endogenous viruses: contains Epstein-Barr
DEPOSITOR: Dr L. Pegoraro, Cytogenetics and Molecular Biology Dept, Turin, Italy
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: The cells express bcl2 and c-myc mRNA
Available in the following LABORATORY:
- IRCCS AOU San Martino -IST Istituto Nazionale per la Ricerca sul Cancro c/o CBA (ICLC, Genova)
RPMI 1640 + 20% FBS + 2mM L-Glutamine; Freezing medium: 90% FBS + 10% DMSO; maintain cultures between 0.5 - 1 x 10^6 cells/ml; 37C, 5% CO2, mycoplasma negative, HOECHST and PCR
the cells grow slowly, are quite difficult to culture and should be frozen at not less than 5 x 10^6 cells/ampoule
Validated by: HLA-DP beta (confirmed as human by PCR)
Availability in cell line catalogues: DSMZ: ACC 20
- J Natl Cancer Inst 1983;70:447-453 - PMID: 6572735
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.