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ICLC catalog code: HTL99014
Continuous culture, grown as monolayer
SPECIES: human, Caucasian (male, 61 years old)
TUMOR: glioblastoma-astrocytoma, grade III
tumorigenic in nude mice
DEPOSITOR: Obtained from ECACC
Cell line not available for distribution.
Available in the following LABORATORY:
- IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
MEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids + 1mM Na pyruvate; Freezing medium: Culture medium + 50% FBS + 10% DMSO; split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 2-4 x 10^4 cells/cm2; 37C, 5% CO2 Passage: 174, mycoplasma negative, HOECHST and PCR
U-251 MG was formerly distributed as U-373 MG until short tandem repeat (STR) genetic profiling confirmed identity with U-251 MG
Validated by: HLA-DP beta (confirmed as human by PCR)
- Acta Pathol Microbiol Scand 1968;74:465-486 - PMID: 4313504
- Hum Hered 1971; 21:238 - PMID: 4332744
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.