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ICLC catalog code: HTL99011
Continuous culture, grown as monolayer
fibroblast-like, cells growing as aggregates and single cells morphology
SPECIES: human, Caucasian (male, 50 years old)
tumorigenic in nude mice
DEPOSITOR: Dr P. Limonta, Institute of Endocrinology, Milan, Italy
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: the cells are reported to produce human prostatic acid phosphatase and prostate specific antigen and to have androgen receptors
Available in the following LABORATORY:
- IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
RPMI 1640 + 15-20% FBS + 2mM L-Glutamine; Freezing medium: 93% FBS + 7% DMSO; split subconfluent cultures 1:2-1:4, do not re-use the same flask; seed at 2-5 x 104 cells/cm2; 37C, 5% CO2 Passage: 33, mycoplasma negative, HOECHST and PCR
the cells are difficult to culture; they grow very slowly, are hard to disaggregate and detach by simply adding PBS; trypsin is used to disaggregate the cells. Thaw the cells with medium supplemented with 20% FBS. More detailed technical instructions will be available on request.
Validated by: HLA-DP beta (confirmed as human by PCR)
Availability in cell line catalogues: IZSBS: BS TCL 148
- Cancer Res 1983;43:1809-1818 - PMID: 6831420
- Models for Prostate Cancer, Alan R. Liss, New York,1981. p.115-132
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.