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ICLC catalog code: ATL98015
Continuous culture, grown as monolayer
SPECIES: mouse, Swiss albino
Original line: NIH/3T3
Transfected by: DNA from rat neuroblastoma B104 Back To Transfection WebPage
DEPOSITOR: Dr U. Pfeffer, Molecular Biology Dept IST at ABC, Genoa, Italy
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: the cells contain the transforming gene neu which induces synthesis of a tumor antigen of relative molecular mass 185,000 (p185), strongly associated with the presence of neuro/glioblastoma oncogenes. The neu oncogene bears homology to erb-B and the tumor antigen p185 is serologically related to the epidermal growth factor receptor
Available in the following LABORATORY:
- IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
DMEM + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; split confluent cultures 1:5-1:10 using trypsin/EDTA; seed at 1-3 x 104 cells/cm2; 37C, 5% CO2, mycoplasma negative, HOECHST and PCR
Validated by: isoenzymes (confirmed as mouse with MD, NP)
- Nature 1984;312:513-516 - PMID: 6095109
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.