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ICLC catalog code: HYL98004
Continuous culture, grown as suspension
SPECIES: mouse, BALB/c
TISSUE/ORGAN: hybrid, B cells x myeloma
DEPOSITOR: Dr T. Florio, Neuroscience Unit, ABC, Genoa, Italy
Cell line available for distribution, only as frozen ampoule. For non-commercial investigative use only
PROPERTIES: fusion partner: SP2/0-Ag14; non producer hybridoma; IL-6 dependent
Available in the following LABORATORY:
- IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
RPMI 1640 + 10% FBS + 2mM L-Glutamine + 50uM 2-mercaptoethanol + 50pg/ml human IL-6 ; Freezing medium: 90% FBS + 10% DMSO; maintain cultures between 3-9 x 10^5 cells/ml; split 1:3-1:4; 37C, 5% CO2, mycoplasma negative, HOECHST and PCR
the cells grow very slowly, particularly after thawing
Validated by: isoenzymes (confirmed as mouse with AST, NP)
Availability in cell line catalogues: DSMZ: ACC 211, ECACC: 96080128
- Eur J Immunol 1987;17:1411-1416 - PMID: 3500054
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.