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ICLC catalog code: ATL98004
Continuous culture, grown as suspension
clusters of small cells morphology
DEPOSITOR: Dr T. Florio, Neuroscience Unit, ABC, Genoa, Italy
Cell line available for distribution upon consent of the depositor. For non-commercial investigative use only
PROPERTIES: the cells are described to synthesize catecholamines (dopamine, norepinephrine); respond reversibly to NGF by induction of the neuronal phenotype.
Available in the following LABORATORY:
- IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
RPMI + 5% FBS + 10% HS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; split 1:3-1:6; seed at 1-2 x 10^5 cells/cm2; 37C, 5% CO2, mycoplasma negative, HOECHST and PCR
to obtain single cells, pass the suspension through a 22G syringe; use collagen-coated flasks for attachment
Availability in cell line catalogues: ECACC: 88022401, DSMZ: ACC 159, IZSBS: BS TCL 91
- Proc Natl Acad Sci USA 1976;73:2424-2426
- Science 1985;229:393-395 - PMID: 3839317
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.