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ICLC catalog code: HTL98009
Continuous culture, grown as suspension
irregularly shaped aggregates morphology
SPECIES: human Karyology: aneuploid: the modal chromosome number tends to decline
TISSUE/ORGAN: lung, pleural effusion
TUMOR: carcinoma, small cell
DEPOSITOR: Obtained from ECACC
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: the cell line has been reported to produce ACTH and related peptides, and calcitonin. HLA class I and II expressed
Available in the following LABORATORY:
- IRCCS AOU San Martino -IST Istituto Nazionale per la Ricerca sul Cancro c/o CBA (ICLC, Genova)
RPMI 1640 + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; split 1:2-1:4; seed at 1-2 x 10^5 cells/cm2; 37C, 5% CO2, mycoplasma negative, HOECHST and PCR
The viability may be low after thawing; thaw the cells with medium supplemented with FBS 20%.
Validated by: HLA-DP beta (confirmed as human by PCR)
- Cancer 1980;45:906-918 - PMID: 6266631
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.