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SPECIES: rat, buffalo
ECACC catalog code: 94121649
Morphology: Flat Epithelial
Description: Rat epithelial cells
Viruses: See Properties
Depositor: Dr M Weiss, Dept. Biologie Moleculaire, Institute Pasteur, Paris, FRANCE (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Feeder cells for co-culture with freshly isolated hepatocytes.
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
Coon's modified Ham's F12 + 2mM Glutamine + 5% Fetal Bovine Serum (FBS). Spit confluent cultures 1:100 i.e. seeding at 1x1,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Karyotype: Diploid or pseudodiploid
Isolated by Dr Hayden Coon. Flat epithelial cell colonies that appear upon low density plating as suspensions of collagenase dissociated buffalo rat liver. Cell origin not clearly defined.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
- Cancer Res (Suppl 17) 1990;5658S-5667S
- Exp Cell Res 1978;113(2):391-402 - PMID: 95676
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.