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TISSUE/ORGAN: kidney, embryonic
ECACC catalog code: 92052131
Description: Human embryo kidney
Viruses: See Properties
Depositor: Dr F L Graham, Department of Biology, McMaster University, Ontario, CANADA (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Products: Adenovirus 5 early region 1(E1) antigens
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
Joklik's MEM + 2mM Glutamine + 10% Horse Serum (HS). Maintain cultures between 5x10,000 to 5x100,000 cells/ml in a Techne stirrer 5% CO2; 37C. Cell doubling time is 30 hours.
Adapted for growth in suspension by passaging 293 cells (ECACC No. 85120602) in nude mice. The cells express adenovirus 5 early region 1(E1) antigens and is permissive for adenovirus 5 host range mutants defective in E1 functions.
Research council deposit: No;
Price Code: B;
Validated by: isoenzymes (species confirmation)
- J Gen Virol 1987;68:937 - PMID: 3819703
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.