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SPECIES: toad, South African
TISSUE/ORGAN: heart, embryonic
DSMZ catalog code: ACC 190
Morphology: large epitheloid (low density monolayer), cobblestone with occasional piling-up (high density monolayer)
Description: toad - South African clawed toad (Xenopus laevis) established from the embryonal (stage 52/53) normal heart endothelium (tadpole heart) of a South African clawed toad (Xenopus laevis Daudin) in 1979; cells were described to synthesize factor-VIII-related antigen (AHG-a.p.), fibronectin and laminin and to be tetraploid (72 +/-5, 2N = 36)
Depositor: Dr. W. K. Schlage, Institute for Biological Research, Koeln, Germany
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% Dulbecco's MEM (diluted with 20% distilled water) + 10% FBS + 20-30 mM HEPES split confluent culture 1:2 every 10-12 days using trypsin/EDTA; avoid centrifugation, just dilute the harvested cells with medium and seed into a new flask; cells grow extremely slowly with a doubling time of about 10-12 days; thaw in cold water (10C), dilute with 10-fold volume of fresh culture medium, centrifuge gently (about 80 x g for 5 min) keep at room temperature in closed flasks (without exposure to CO2 ) cell harvest of about 2.5 x 106 cells/80 cm 2;
doubling time of ca. 10-12 days;
frozen with 90% complete medium + 10% glycerol (not DMSO) at about 0.5 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization assays;
- Cell Tissue Res 1986;245:445-451 - PMID: 3091251
- Eur J Cell Biol 1981;24:21A - PMID: 7327181
- Microsc Acta 1983;87:19-34 - PMID: 6828001
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.