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TISSUE/ORGAN: lung, endothelium
ECACC catalog code: 86123101
Susceptibility to viruses: bovine respiratory
Description: Bovine lung endothelium
Viruses: See Properties
Depositor: Dr A Hussein, Dept. of Paediatrics, John Radcliffe Hospital, Oxford, UK
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Virus studies: bovine respiratory viruses
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM or 199 + 2mM Glutamine + 15% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 to 1:4 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
BPAEC is susceptible to bovine respiratory viruses and is positive for BVDV.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.