This is the complete description of the cell line that you selected.
By clicking on a generic term (e.g., SPECIES) you will go to the corresponding index (e.g., index of species), while by clicking on a specific term (e.g., retinoblastoma) you will go to the list of corresponding cell lines (e.g., retionoblastoma cell lines).
SPECIES: rat, Wistar
DSMZ catalog code: ACC 118
Morphology: epithelial-like, small cells growing as monolayers
Endogenous viruses: contains Rous sarcoma virus
Susceptibility to viruses: Moloney-MuLV * vesic. stomatitis (Indiana)
Description: rat sarcoma the original tumor was induced in a Wistar rat by intramuscular injection of Rous sarcoma virus; cells have been used as indicator cells for detection of murine leukemia viruses confirmed as rat with IEF of AST, NP
Viruses: ELISA. reverse transcriptase positive
Depositor: obtained from ATCC (CCL 165), Rockville, Maryland, USA
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% MEM (with Earle's salts) + 10% FBS + non-essential amino acids split confluent culture 1:4 every 3 days using trysin/EDTA at 37C with 5% CO2 about 1-2 x 107 cells/80 cm 2;
frozen with 70% medium, 20% FBS, 10% DMSO at about 2-3 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * isoenzymes (confirmed as rat with IEF of AST, NP)
Availability in cell line catalogues: ATCC: CCL 165, IZSBS: BS TCL66, ECACC: 88120601, ICLC: ATL96013
- Folia Biol (Praha) 1962;8:221
- Nature 1960;186:980-981
- Proc Natl Acad Sci U S A 1969;63:753-758 - PMID: 4186808
- Virology 1970;42:1136-1139 - PMID: 4099080
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.