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TUMOR: lymphoma, B cell
DSMZ catalog code: ACC 58
Morphology: round to elongated cells in suspension, single or in small clusters
Description: human B cell lymphoma established from the pleural effusion of a 46-year-old woman with nodular histiocytic lymphoma (refractory, progressive) in 1986; reported to carry the 14;18 translocation and to express mRNA for the bcl-2 oncogene confirmed as human with IEF of MDH, NP, PEP B
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. A. Al-Katib, Wayne State University, Detroit, Michigan, USA
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS maintain at 0.5-2.0 x 106 cells; split 1:2 to 1:4 every 2-4 days; seed out at ca. 0.5-0.8 x 106 cells/ml at 37C with 5% CO2 maximum density at about 2.5 x 106 cells/ml;
doubling time of ca. 45 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule;
negative in DAPI, microbiological culture, PCR assays;
Validated by: cytogenetics 1 (hypodiploid with 6% polyploidy, spontaneous aberrations frequent; 45(42-45)<2n>X, -X, dup(7)(q21qter), der(8)t(8;?)(p11;?), der(11)t(1;11)(q24;p15), der(12)t(12;?)(p11;?), der(17)t(17;?)(p12;?), der(18), t(?14;18)(q32;q21). der(1 )absent) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (CD3- CD13- CD19+ CD37- HLA-DR- smIgG+ smIgM-) * isoenzymes (confirmed as human with IEF of MDH, NP, PEP B) * reverse transcriptase (reverse transcriptase not detected)
- Leuk Res 1988;12:833-843 - PMID: 3143865
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.