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TUMOR: leukemia, hairy cell
DSMZ catalog code: ACC 150
Morphology: lymphoblast-like cells growing as single cells and in clumps in suspension
Description: human hairy cell leukemia established from the spleen of a 46-year-old man with hairy cell leukemia (HCL) at diagnosis in 1988 confirmed as human with IEF of AST, MDH
Viruses: ELISA: reverse transcriptase negative; PCR: EBV+, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. J. C. Kluin-Nelemans, Acad. Ziekenhuis, Leiden, The Netherlands
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS maintain at 0.5-1.0 x 106 cells/ml; seed out at ca. 0.5 x 106 cells/ml at 37C with 5% CO2 saturation density at about 2.0-2.5 x 10 6 cells/ml;
doubling time of ca. 50-60 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (human hyperdiploid with 4% polyploidy; supernumerary ring or acf present in most cells; 47(45-47)<2n>X, -Y, +12, t(9;10)(p12-13;q12)) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (CD3- CD10- CD13- CD19+ CD20+ CD37+ HLA-DR+ cy/smIgM+ cy/smIgG- cy/sm_+ cy/sm_-) * isoenzymes (confirmed as human with IEF of AST, MDH) * reverse transcriptase (negative)
- Leuk Lymphoma 1991;5:415-422
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.