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ECACC catalog code: 93120839
Description: Human conjunctival D cell, HeLa characteristics
Viruses: See Properties
Depositor: Dr M Ferrari, Instituto Zooprofilattico, Brescia, ITALY
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Virus studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
Medium 199 (HBSS) + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37C.
Karyotype: 2n = 46
Derived from normal human conjunctiva. The cells support the growth of influenza A-B virus, poliovirus 1, VSV (Indiana strain) and influenza. This cell line was found to have a HeLa profile by DNA fingerprinting.
Research council deposit: No;
Price Code: C;
Validated by: DNA fingerprinting (HeLa profile) * isoenzymes (species confirmation)
- Proc Soc Exp Biol Med 1954;87:440
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.