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ECACC catalog code: 88102403
Susceptibility to viruses: adenovirus 3 * measles * poliovirus 1 * poliovirus 2 * vesic. stomatitis (Indiana) * vesicular stomatitis
Description: Human amnion, HeLa characteristics
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Virus studies: VSV (Indiana), Ad 3, Poliovirus, measles virus
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 15% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 to 1:10 i.e. seeding at 1-5x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37C.
Karyotype: 2n = 46
From human amnion tissue. Contaminated with HeLa cells. Have been used in virus studies; susceptible to VSV (Indiana), adenovirus 3 and poliovirus. Useful in differentiating virulent and avirulent measles virus. HeLa marker chromosomes and type A G6PD This cell line was found to have a HeLa profile by DNA fingerprinting.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 25, IZSBS: BS CL92
- Am J Dis Child 1962;103:460
- Exp Cell Res 1961;23:14
- Nature 1976;259:211-213 - PMID: 1250349
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.