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SPECIES: human, Caucasian
TISSUE/ORGAN: lung, embryonic
ECACC catalog code: 89101301
TRANSFORMED BY: SV40
Endogenous viruses: contains SV40
Susceptibility to viruses: herpes simplex * poliovirus 2 * Sindbis * vesic. stomatitis (Indiana)
Description: Human Caucasian fetal lung, SV40 transformed
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Transformation and virus studies: SV40
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 10% Fetal Bovine Serum (FBS) + 2mM Glutamine. Split confluent cultures 1:2 to 1:5 i.e. seeding at 2-5x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37C.
Karyotype: 2n = 46, unstable karyotype
An SV40 transformed derivative of WI-26, a human diploid cell line derived from embryonic lung tissue of a male Caucasian. The cells have SV40 T-Ag but infectious virus has not been rescued.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 95.1
- Exp Cell Res 1961;25:585-621
- Exp Cell Res 1965;37:614-636
- J Cell Comp Physiol 1965;65:69-84 - PMID: 5863367
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.