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SPECIES: tick - boophilus microplus, paquera
ECACC catalog code: 94022804
Morphology: Haemocytic, undifferentiated, adherent and large round floating granular cells.
Description: Tick, Boophilus microplus, eggs
Viruses: See Properties
Depositor: Dr L Bell-Sakyi, Centre for Tropical Medicine, University of Edinburgh, UK (Originator)
No restrictions. Patent: None Specified By Depositor
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
L15:EMEM (HBSS) (1:1) + 2mM glutamine + 0.1% bovine plasma albumin + 10% TPB + 20% FBS. Split confluent cultures 1:1 to 1:3 once a week. Do not exceed 28C incubation temperature. Change media weekly by removal and replacement of half 4.5ml total vol. No C02 required
Derived from Boophilus microplus Paquera strain embryo (eggs). This species is acancide susceptible. In continuous culture since 1983.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
- Cen Trop Vet Med 1985/1986;Ann Rep
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.