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ECACC catalog code: 95082908
TRANSFORMED BY: Marek's disease virus
Description: Mouse fibroblast, WOP derivative
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Packaging system for recombinant retroviral vectors
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 250µg/ml Xanthine +15µg/ml Hypoxanthine + 10µg/ml Thymidine + 2µg/ml Aminopterin + 15µg/ml Mycophenolic acid + 10% Fetal Bovine Serum (FBS); adjust pH before use. Split confluent cultures 1:4 to 1:8 i.e. seeding at 1-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Wgd5 cells were derived from the WOP cell line which is a 3T3 derivative transformed by an origin-defective polyomavirus. The Wgd5 cell line contains a mutant of the Moloney murine leukaemia virus (MoMuLV) and was selected as a gpt+ clone after co-transformation of WOP with pSV2gpt and a packaging mutant of MoMuLV.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CRL 1817
- Proc Natl Acad Sci U S A 1987;84:8277 - PMID: 3479791
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.