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SPECIES: rat, Sprague-Dawley
TUMOR: sarcoma, osteogenic
ECACC catalog code: 91060527
Description: Rat Sprague-Dawley osteosarcoma
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Hormone studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
A cloned derivative of a transplantable rat osteogenic sarcoma. Cells retain many characteristics of the tumour including responsiveness to parathyroid hormone, cytosol receptors for the bone resorbing steroid hormone 1,25-OH)2D3 and prostaglandins. Similar to cell line UMR-106.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CRL 1663
- Endocrinology 1977;101:555-562 - PMID: 195798
- J Biol Chem 1980;255:4414-4417 - PMID: 6929275
- Nature 1976;260:436-438 - PMID: 1062678
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.