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ICLC catalog code: HTL94002
Continuous culture, grown as suspension
SPECIES: human, Caucasian (male, 37 years old)
TISSUE/ORGAN: pleural effusion
TUMOR: lymphoma, histiocytic
Susceptibility to viruses: HIV-1 * HIV-2
DEPOSITOR: Obtained from ATCC. Established by C. Sundrom and K. Nilsson in 1974.
Cell line available for distribution. The cell line must not be exploited commercially
PROPERTIES: IL-1 production; specialized product secretion; tumor markers; Fc receptors; antitumour testing; virology; tumorigenicity; expression of markers and properties of monocytes, expression of mRNA for c-myc.
Available in the following LABORATORY:
- IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
RPMI 1640 + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Split 1:5-1:20, maintain cultures between 2 and 9x105 cells/ml. 37C, 5% CO2., mycoplasma negative, HOECHST and PCR
Validated by: HLA-DP beta (confirmed as human by PCR) * identification confirmed by Short Tandem Repeat (STR) profile (Amelogenin: X; vWA: 14,15,16,17; FGA: 22,25; TH01: 6,9.3; D18S51: 13,14; D21S11: 27,29; D8S1179: 12,13) * isoenzymes (confirmed as human with AST, NP, PepB)
Molecular authentication: Amelogenin: X ; D18S51: 13,14 ; D21S11: 27,29 ; D8S1179: 12,13 ; FGA: 22,25 ; TH01: 6,9.3 ; VWA: 14,15,16,17
Availability in cell line catalogues: DSMZ: ACC 5
- Int J Cancer 1976;17:565-577 - PMID: 178611
- J Exp Med 1976;143:1528-1533 - PMID: 1083890
- J Immunol 1980;125:463-465 - PMID: 7381208
- Nature 1979;279:328-331 - PMID: 450085
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.