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SPECIES: human, Caucasian
TUMOR: lymphoma, Burkitt
DSMZ catalog code: ACC 233
Morphology: single, round cells growing non-adherent in suspension
Description: human Burkitt's lymphoma established from the tumor tissue at relapse of a 16-year-old Caucasian boy with Burkitt's lymphoma; cells were described to be EBV-negative confirmed as human with IEF of MDH, NP
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HIV-, HTLV-I/II-
Depositor: Dr. M. H. Falk, GSF, Institut for Clinical Molecular Genetics and Tumor Genetics, Munich, Germany
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS (possibly add 1 mM sodium pyruvate + 50 śM mercaptoethanol) optimal split ratio of 1:2 to 1:3 every 2-3 days at 37C with 5% CO2 saturation density of about 1.0 x 106 cells/ml;
doubling time of about 44 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (human hyperdiploid karyotype with 4% polyploidy; 47(42- 49)<2n>XY, +7, inv(1)(p21q21), del(2)(q33), t(8;14) (q24;q32), t(12;22)(q21.1;q13.2)) * DNA fingerprinting (unique, homozygous profile with pYNH24 probe) * immunological analysis (CD3- CD10- CD13- CD19(+) CD20+ CD37+ HLA-DR+ cy/sIgM+ cy/sIgG- cy/sIgk+ cy/sIglamda-) * reverse transcriptase (reverse transcriptase not detected)
- IARC Scientific Publ 1985;60:309-318 - PMID: 3934070
- J Natl Cancer Inst 1987;78:235-242 - PMID: 3027441
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.