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TUMOR: lymphoma, B cell
DSMZ catalog code: ACC 4
Morphology: single or clumped cells in suspension; some cells adhere to plastic
Description: human B cell lymphoma established from the involved tonsil of a 7-year-old boy with lymphoblastic lymphoma at diagnosis prior to therapy in 1972; cells were described to be EBV-negative confirmed as human with IEF of AST, NP
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. K. Nilsson, University Hospital, Uppsala, Sweden
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS maintain at 0.2-0.7 x 106 cells/ml; split 1:2 to 1:5 every 2-3 days; some cells adhere to plastic, but can be detached mechanically (trypsin not necessary); seed out at ca. 0.5 x 106 cells/ml at 37C with 5% CO2 maximal density at 1.5 x 106 cells/ml;
doubling time of ca. 48 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 4 x 106 cells/ampoule;
negative in DAPI, microbiological culture assays;
Validated by: cytogenetics 1 (hyperdiploid with 6% polyploidy; 49(46-50)<2n>XY, +6, +7, +m, der(3)t(3;?)(q11;?), del(6)(q24)) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (CD3- CD13- CD19+ CD37+ HLA-DR+) * isoenzymes (confirmed as human with IEF of AST, NP)
- Int J Cancer 1974;13:808-823 - PMID: 4136723
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.