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SPECIES: human, Caucasian
TUMOR: lymphoma, Burkitt
DSMZ catalog code: ACC 160
Morphology: single, round cells growing non-adherent in suspension
Description: human Burkitt's lymphoma established from the tumor tissue of an 8-year-old Caucasian boy with Burkitt's lymphoma; cells were described to be EBV-negative confirmed as human with IEF of AST, NP
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. M. H. Falk, GSF, Institute for Clinical Molecular Genetics and Tumor Genetics, Munich, Germany
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90-95% RPMI 1640 + 5-10% FBS (possibly add 1 mM sodium pyruvate + 50 śM mercaptoethanol) split saturated culture 1:3 to 1:5 every 2 days; optimal density at about 1.0 x 106 cells/ml at 37C with 5% CO2 saturation density at about 2.0 x 106 cells/ml;
doubling time of ca. 30 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 4 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (human hyperdiploid karyotype with 7% polyploidy; 48(42- 49)<2n>XY, +7, -13, +2mar, add(8)(q24), t(8;14)(q24;q32), der(15)t(13;15)(q13;p11), add(17)(p12), subclonal rearrangements at 1q23, 7p22, 11q13) * DNA fingerprinting (unique, heterozygous profile with pYNH24 probe) * immunological analysis (CD3- CD10+ CD13- CD19+ CD20+ CD37+ HLA-DR+ cy/sIgM+ cy/sIgk+) * isoenzymes (confirmed as human with IEF of AST, NP) * reverse transcriptase (reverse transcriptase not detected)
- IARC Scientific Publ 1985;60:309-318 - PMID: 3934070
- J Natl Cancer Inst 1987;78:235-242 - PMID: 3027441
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.