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TISSUE/ORGAN: peripheral blood
TUMOR: multiple myeloma
DSMZ catalog code: ACC 9
Morphology: round to polygonal, single or clustered cells in suspension, some loosely adherent
Description: human multiple myeloma established from the peripheral blood of a 53-year-old man with IgE-secreting myeloma (refractory, terminal) in 1968; cells were described to produce IgE lambda; possible fusion partner for hybridoma production; cells express mRNA for bcl-2 gene confirmed as human with IEF of AST, MDH, NP
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HIV-, HTLV-I/II-
Depositor: Dr. K. Nilsson, University Hospital, Uppsala, Sweden
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS maintain at 0.2-1.0 x 106 cells/ml; cells grow partially (loosely) adherent and can be removed with a bent Pasteur pipette (trypsin is not necessary); seed out at ca. 0.5-0.7 x 106 cells/ml at 37C with 5% CO2 saturation density at 1.0-1.5 x 106 cells/ml;
doubling time of ca. 55 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule;
contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, PCR assays;
Validated by: cytogenetics 1 (hypodiploid karyotype with 7% polyploidy; 44(40-46)<2n>XY, -8, -10, -17, -18, -20, -22, +4mar, der(1)t(1;11)(p33;q13), der(3)t(3;?)(q27;?), t(4;11)(q?21;q?22), dup(7)(q32q34), der(8)t(8;?)(q24;?), der(10)t(10;?)(p12;?), der(14)t( 4;?)) * cytogenetics 2 ((p11;?), der(15)t(15;?)(q26;?)) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (CD3- CD5- CD10- CD13- CD19- CD21- CD22- CD37- CD38+ HLA-DR+ smIgG- smIgM- cy_+ cy_-) * isoenzymes (confirmed as human with IEF of AST, MDH, NP)
Availability in cell line catalogues: ATCC: TIB 196
- Clin Exp Immunol 1970;7:477-489 - PMID: 4097745
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.