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TUMOR: lymphoma, Hodgkin
DSMZ catalog code: ACC 72
Morphology: round cells growing singly in suspension
Description: human Hodgkin's lymphoma established from the bone marrow of a 20-year-old woman with Hodgkin's disease (nodular sclerosis; stage IVB, pre-terminal stage) confirmed as human with IEF of AST, LDH
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. H. G. Drexler, DSMZ, Braunschweig, Germany
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
80% RPMI 1640 + 20% FBS split saturated culture 1:2 every 3 days, exchange spent medium every 1-2 days; seed out at ca. 0.5 x 106 cells/ml; after thawing culture first in 24-well-plates at 37C with 5% CO2 saturation density at about 1.5 x 106 cells/ml;
doubling time of ca. 70 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule;
contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (pseudodiploid with 1.5% polyploidy; 46(44-46)<2n>XX, + 7, -9, der(7)t(7;?)(p21;?), der(8)t(2;8)(p12;q24)) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (CD3- CD10+ CD13- CD19+ CD37+ HLA-DR+ smIgG- smIgM+ sm_+ sm_-) * isoenzymes (confirmed as human with IEF of AST, NP, PEP B) * reverse transcriptase (negative)
- Biomedicine 1979;22:276-284
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.